|
|
Single
bronchoscopy protocol |
Longitudinal
bronchoscopy protocol |
Burn
patient protocol |
Respiratory
failure patient protocol |
|
Participant type(s) |
AUD and/or chronic cannabis participants and pair-matched controls |
AUD participants |
Burn ICU patients, � AUDs |
Respiratory failure patients in ICU, � AUDs |
|
Demographic, AUD, smoking (cigarettes and/or cannabis) data collection |
Yes, from participant |
Yes, from participant |
Yes, from healthcare proxy, confirmed by patient |
Yes, from healthcare proxy, confirmed by patient |
|
Length of protocol |
1 day inpatient (controls); 2-3 days inpatient (AUDs) |
11 days (8 days inpatient, 3 days outpatient) |
Varies (inpatient) |
Varies (inpatient) |
|
Blood (plasma, serum, buffy coat, peripheral blood mononuclear cells) |
Day 1 |
Days 1, 4, and 7 |
Within 24 hours of injury, Day 3-5, Day 8-10 |
Within 24 hours of mechanical ventilation, Day 3-5, and Day 8-10 |
|
Bronchoscopic samples |
BAL, cytologic brushings x3 on Day 1 |
BAL, cytologic brushings x3 on Days 1 and 7 |
BAL, mini-BAL, or tracheal aspirate <48 hours post-intubation |
BAL, mini-BAL, or tracheal aspirate <48 hours post-intubation |
|
Other sample types |
Nasal epithelial brushing on Day 1; urine collection on Day 1 |
Nasal epithelial brushing on Day 1; urine collection on Day 1 and Day 7 |
None |
Urine collection on Day 1 and Day 8-10 |
|
Blood volume at each time point for single bronchoscopy protocol and longitudinal bronchoscopy protocol = 35cc. Blood volume at each time point for respiratory failure patients in the ICU = 10cc. Blood volume at each time point for burn patients in the ICU = 5cc. BAL yield for ambulatory participants = up to 175-200cc (depending on volume of saline used) with 10-20 million WBCs; for critically ill patients = 10-20cc with 10-20 million WBCs. Mini BAL yield = 1-4cc with 1-10 million WBCs. |
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