Customer is responsible for sample homogenization and BCA assay to determine protein content for normalization purposes. The NORC Lipidomics Laboratory staff extracts the samples, runs the quantitative LC-MS/MS assays, and analyzes the lipidomic data.

All samples need to be delivered or shipped in a -80C freezer box in the order they are listed on the . Sample tubes MUST be numbered sequentially (1 to x, where is x is your last sample).  Any unique code for your samples can be recorded on the Sample Inventory Form in the sample description column.

All academic analyses are subsidized by the 麻豆传媒高清 NORC. Reduced pricing is available for junior investigators. Turnaround time is dependent on the number of samples submitted, the specific lipids of interest, and the number of samples in our queue.  For current pricing and to discuss sample analysis and turnaround time, please contact Karin Zemski-Berry. 

All samples need to be delivered or shipped in a -80C freezer box in the order they are listed on the .  Sample tubes MUST be numbered sequentially (1 to x, where is x is your last sample).  Any unique code for your samples can be recorded on the Sample Inventory Form in the sample description column. The NORC Lipidomics Laboratory staff processes the sample, runs the GC/MS assay and analyzes the data. 

All academic analyses are subsidized by the 麻豆传媒高清 NORC. Reduced pricing is available for junior investigators. Turnaround time is dependent on the number and types of samples to prepare and run, as well as the amount of samples in our queue. For current pricing and to discuss sample analysis and turnaround time, please contact Karin Zemski-Berry. 

Both a XFe96 Seahorse and a pair of O2k Respirometers are available for respirometry assays in intact cells, permeabilized cells, and isolated mitochondria. These assays can be used to quantify mitochondrial ATP production, maximal capacity of the respiratory chain, uncoupling, mitochondrial membrane potential, and ROS production. 

Normalization of the XFe96 Seahorse data via cell number, genomic DNA, and total cellular protein, can be a time consuming processes. To facilitate a simple, time saving solution to normalization, the NORC MCA core has purchased a BioTek Cytation-1 Cell Imaging Multi-Mode Reader to work in concert with the XFe96 Seahorse for normalization purposes. Following a Seahorse assay run, Hoechst stain is added, and the Seahorse plate is then transferred to the Cytation-1 imager. Images and counts are then transferred back into the Wave software for normalization of the Seahorse ECAR and OCAR data. The entire normalization procedure takes approximately 30 minutes.

Both a XFe96 Seahorse and a pair of O2k Respirometers are available for respirometry assays in intact cells, permeabilized cells, and isolated mitochondria. These assays can be used to quantify mitochondrial ATP production, maximal capacity of the respiratory chain, uncoupling, mitochondrial membrane potential, and ROS production. 

Allows for the simultaneous monitoring of oxygen consumption together with either ROS production or mitochondrial membrane potential.

Both a XFe96 Seahorse and a pair of O2k Respirometers are available for respirometry assays in intact cells, permeabilized cells, and isolated mitochondria. These assays can be used to quantify mitochondrial ATP production, maximal capacity of the respiratory chain, uncoupling, mitochondrial membrane potential, and ROS production. 

Coupled to a 782 oxygen meter.

The Jess is a fully automated capillary-based immunoassay method, requiring less sample, antibody, and time compared to traditional western blots. Simply pipette samples and reagents into the microplate, and the machine automatically separates proteins by size, manages antibody additions, incubations, washes, and detection. Up to 25 samples may be run in approximately 3hrs.  Multiplexing is also possible, as the Jess allows for 4 different ways to analyze proteins, Fluorescent (IR and NIR), chemiluminescent, and protein normalization.